Journal: The Journal of Biological Chemistry

Article Title: The permanently chaperone-active small heat shock protein Hsp17 from Caenorhabditis elegans exhibits topological separation of its N-terminal regions

doi: 10.1016/j.jbc.2022.102753

Figure Lengend Snippet: Negative-stain EM of aggregation assay samples with different substrates. Micrographs of Hsp17 with CS ( top row ), MDH ( second row ), and insulin ( bottom row ) as substrates in chaperone activity assays. The first two columns show Hsp17-only and substrate-only controls. Columns three and four display the endpoints of the activity assays with increased Hsp17 ratios. The same Hsp17 concentrations as in the corresponding chaperone activity assays were used. Samples were taken either after 30 min of incubation (CS and MDH) or 75 min (insulin). Additional time points and concentrations have been analyzed for the chaperone assay with insulin, see Fig. S8 . Micrographs were acquired at 10k ( upper images ) and 60k ( lower images ) magnification. The areas in red boxes show the same part of the sample. Scale bars represent 500 nm and 50 nm, respectively. For each state, two independently prepared grids and at least 5 gridsquares per grid were analyzed. The chosen images represent the typical view during the analysis. MDH, malate dehydrogenase; CS, citrate synthase.

Article Snippet: Immediately after the incubation step, sample buffer for clear native gels (Serva) was added, and samples were loaded on 4 to 16% native gels (Serva) and separated using native anode and cathode buffers from Serva.

Techniques: Staining, Activity Assay, Incubation

Journal: Cell reports

Article Title: Tentonin 3 is a pore-forming subunit of a slow inactivation mechanosensitive channel

doi: 10.1016/j.celrep.2024.114334

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: T/G Native Sample Buffer , Koma Biotech , Cat# KTG020N.

Techniques: Recombinant, Modification, Purification, Mutagenesis, Sample Prep, Software